1. Purpose: To determine if the "ebony" body color and "curved" wing alleles assort independently.

Cross No. 1
a) Cross ebony x curved . Record your observations.
b) What does this tell you about dominance of curved and ebony?
c) Cross the F1 x F1 . Record your observations.
d) Calculate the recombination frequency between ebony and curved.

Cross No. 2
e) Cross ebony, curved x wt . Record your observations.
f) Cross the F1 x ebony, curved parent (this is a "backcross"). Record your observations.
g) Calculate the recombination frequency between ebony and curved.

Cross No. 3
h) Cross wt x ebony, curved . Record your observations.
i) Backcross the F1 x ebony, curved parent.
j) Calculate the recombination frequency between ebony and curved.

Comparing the three procedures:
k) Are the recombination frequencies in (d) and (g) and (j) the same? Explain any differences.
l) What do the data tell you about independent assortment?
m) Which is the best procedure for testing independent assortment? Why?

2. Purpose: To determine if the "curved" wing and "purple" eye color alleles assort independently.
a) Based upon your evidence in question 1, choose a procedure and set up an experiment to test independent assortment of curved and purple. Record your observations.
b) What does your experiment tell you about dominance of purple and curved?
c) Calculate the recombination frequency between curved and purple.
d) What do the data tell you about independent assortment?