Histology                                                                                                         Stanley Shostak
BioSc 1450                                                                                                      Spring 05

Lecture 7. Integumentary System = Skin

Basic Structure

External surface: keratinized squamous epithelium = epidermis; embryological source of epidermal appendages

Supported by: thick, dense, fibro-elastic CT = dermis; highly vascular & contains sensory receptors; e.g., pressure receptors (Pacinian corpuscles).

Underlying layer: hypodermis or subcutaneous layer; loose CT; variable amâts adipose tissue; = superficial fascia

Epidermis

Thick skin: palms of hands and fingers; soles of feet

downward folds: epidermal or rete ridges (note: ret- = net) between dermal pegs or papillae in papillary zone of dermis (see below)

keratinocytes: lineage differentiating cells; 25 to 50 days course of differentiation

melanocytes: usually confined to basal layer; have long dendritic processes; ramify; do not establish cell junctions; cell bodies located btwn basal epi cells and basement mem. premelanosomes mature to melanosomes; transferred to keratinocytes; synthesis promoted by melanocyte stimulating hormone (MSH) from pituitary

tactile disc: Merkel cells: associated with free nerve endings; may be  part of diffuse neuroendocrine system

dendritic cell = Langerhan's cells: macrophage-monocyte; antigen presenting cell (= APC); throughout lower epidermal layers; long dendritic processes; atopic and other immunologically based skin disorders; stimulated: migrate via dermal lymphatic to paracortical zones of regional lymph nodes;

5 morphological layers

stratum germinativum or basale (= stratum malpighi): germinal layer: adjacent to dermis
stratum spinosum or prickle cell layer: growth and early keratinization; prickles = desmosomes; prominent nucleoli and cytoplasmic basophilia indicate protein synthesis; cytokeratin: (various types) predominant synthetic product; aggregates in intracellular fibrils = tonofibrils; converge on desmosomes;
stratum granulosum or granular layer: intracellular granules
stratum lucidum (only extremely thick skin): homogeneous
stratum corneum or cornified layer: flattened, fused cell remnants; outer keratinized layer; shed continuously, replaced from below
 
Thin skin: stratum corneum reduced; lucidum and granulosum virtually gone, spiosum reduced; rete ridges less prominent.

Dermis

mainly fibroblasts plus usual ct elements; two zones

papillary dermis: upward projections: dermal papillae and immediate adjacent CT; loose, highly vascular, fine interlacing collagen fibers; fine interlacing network fiber of elastin  
subpapillary arterial, venous and lymphatic plexuses and papillary vascular loops
fine axonal connections of free sensory nerve endings (to epidermis)

corpuscle of touch = Meissner's corpuscles
lamellated corpuscle = deep pressure receptor = Pacinian corpuscle
superficial touch receptor = Meissner's corpuscle
 


reticular layer: dense, irregular ct: interlacing arrangement of collagen fibers; coarse, disposed in thick irregular bundles; contains blood vessels to subpapillary plexuses from hypodermal cutaneous plexuses; elastin in long thick fibers

Skin appendages: epidermal extensions

Hairy (versus glabrous [hairless and thick]) regions scalp: robust, thick, densely collagenous dermis; hair follicles long and have numerous sebaceous glands, arrector pilae, closely packed

body surface: merocrine sweat glands short; hair follicles associated with sebaceous glands sparse; finer hairs

axillae and pubic region: hair oriented obliquely to skin surface, often curved rather than straight ÷ curled hair; apocrine sweat glands common; associated with hair follicles

hair follicle: shaft & bulb; growth cycle: growing phase, resting phase, club hair. bulb: whole epithelial mass surrounding dermal papilla
shaft: contains hair; 4 keratinizing concentric layers formed by bulb; + outermost layer (external root sheath) nonkeratinizing downward extension of stratum germinativum of epidermis inner 3 keratinizing layers = hair 1) medulla: core of hair shaft; not distinguished in fine hair
2) cortex: bulk of hair
3) cuticle: hard, thin, surface of overlapping plates; prevent matting anchor hair
 
outer 2 layers = root sheath 4) internal root sheath (& cuticle): lightly keratinized; disintegrates at level of sebaceous glands
5) external root sheath: not keratinized; not part of hair formation; separated from CT by glassy membrane
 
hair bulb: epithelial layers (1 to 4) merge; hair matrix = mass of cells destined to form hair plus inner root sheath; encloses dermal papilla; keratinocytes & melanocytes
 
vellus: infants, children, females: body hair fine and soft;   terminal hair: coarser hair of scalp; male sex hormones produced at puberty cause development of further terminal hair in pubic and axillary regions of both sexes and replacement of vellus with terminal hair on male face   dermal papilla: formed by neural crest cells; melanocytes abundant; nerve endings

arrector pili: bundle smooth muscle fibers; insert on sheath below sebaceous gland and on dermal papillary area beneath epidermis

innervated by sympathetic nervous system; activated by cold or fear; expulsion of sebum from sebaceous glands
 
Sebaceous glands: holocrine secretion (of cells near duct) Pilosebaceous unit: outgrowths of external root sheath; lie within CT sheath of hair; branched acinar form with; short duct; lipid-filled vacuoles (poorly stained cytoplasm); mitosis in basal layer of acini secrete sebum:
also independent of hair follicle in transition regions: lips, eyelids, glans penis, labia minora & nipples
 
Sweat glands also glands of Moll (margins of eyelids) & wax (ceruminous) glands of external auditory canals; generally simple, coiled tubular glands; secrete watery fluid merocrine sweat glands: innervated by cholinergic fibers; stimulated by excessive body heat, fear-provoking stimuli; Note: absent margins of lip and glans penis
secretory portion: merocrine = eccrine secretion; hypotonic with respect to plasma; contains Na+ and Cl- other ions urea & sm mol. wt. metabolites (a minor mode of excretion); myoepithelial cells surrounding secretory portion; dark (may secrete glycoprotein) and clear secretory cells (secrete Na+; water follows)
excretory portion: stratified cuboidal epithelium; narrower lumen; no myoepithelial cells; resorbs Na+; may resorb glycoprotein.
sweat pore: located on epidermal ridge
 
apocrine sweat glands: odiferous glands & mammary glands (modified):areolae of breasts, axilla and genital regions; apocrine secretion; viscid, milky secretion discharged into hair follicles; becomes 'malodorous' after action of commensal bacteria; become functional after puberty
larger glands; lumen of secretory portion dilated; low cuboidal cells; eosinophilic cytoplasm; discontinuous layer of myoepithelial cells beneath basement membrane

innervated by adrenergic fibers of sympathetic nervous system.

 
fingernail: nail plate: dense keratinized plate; slides distally over rest of nail bed hyponychium: skin beneath free end of nail plate nail bed: stratified squamous epithelium; does not contribute actively to nail growth
nail root: proximal to nail bed; firmly attached to distal interphalangeal joint and periosteum of distal phalanx nail matrix: thickened epithelium underlying nail root; pronounced epidermal ridges; proliferation and differentiation produces nail growth;
lunular: white crescent shaped area at base of nail; surface appearance distal part of nail matrix
nail fold: skin overlying root of nail;
eponychium: highly keratinized free edge of nail fold
 
Skin circulation: adaptations to heat flow (loss)
  plexuses of anastomosing vessels: cutaneous plexus: deeper plexus; lies at junction of hypodermis and dermis; supply fatty tissue of hypodermis; deeper aspects of dermis & capillary networks enveloping hair follicles, deep sebaceous glands, sweat glands

subpapillary plexus: superficial plexus beneath dermal papillae; upper aspects of dermis and capillary networks around superficial appendages; capillary loop in each dermal papilla

arterio-venous shunts: numberous; control blood flow

glomus body: dermis finger tips and peripheral sites prone to excessive cold; highly convoluted segment of arterio-venous shunt enveloped by condensed collagenous tissue; thickened (smooth muscle?) wall of artery before junction with vein assumes epithelioid appearance

similar lymphatic drainage

last revised: 02-01-05