�Margaret Dayhoff

�Atlas of Protein Sequences

�Phylogenies, evolution, amino acid substitution matrices (PAM) and discovering active sites in enzymes

�PIR SF Evolutionary Family , iProClass Functional site analysis and ontologies , iProLink to literature

�UniProt - Universal Protein Resource

�Amos Bairoch

�Manual curation and annotation

�Highly cross-referenced

�Many useful analytical tools (EXPASY Tools)

�2D-PAGE and Mass Spectrometry databases

�Prosite functional motif database

�UniProt - Universal Protein Resource

�Translation of mRNAs (RefSeq), UniGene, open reading frames (ORFs) and predicted genes from genomes

�Automatic annotations

�Clusters

�Interpro linked to domain and motif databases (CATH , PANTHER , PRINTs, PROSITE, pFAM, PIRSF, PRODOM, SCOP, SMART, SUPERFAMILY)

�Intron-exon structure and links to ORFs, coding regions

�UniProt - Universal Protein Resource

�Protein and nrPRO database SwissProt, PIR and translated genes/genomes

�Protein Clusters Database (prokaryotic) and COGS and KOGS

�Linked to coding regions and intron/exon structure

�Linked to coding SNPs and variations databases

�Linked to MMDSB structure database

�Linked to 3D domains

�Linked to CDD Conserved Domain Database