Boyle Lab  
Department of Biological Sciences
University of Pittsburgh


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2015 Wier GM, McGreevy EM, Brown MJ, Boyle JP.  New Method for the Orthogonal Labeling and Purification of Toxoplasma gondii Proteins While Inside the Host Cell. (2015)  MBio 6(2). pii: e01628-14. doi: 10.1128/mBio.01628-14.  Link to open access article.
Review:  English ED, Adomako-Ankomah Y, Boyle JP.  Secreted effectors in Toxoplasma gondii and related species: determinants of host range and pathogenesis? (2015)  Parasite Immunol. 37(3):127-40. doi: 10.1111/pim.12166.  Link to open access article.
2014 Walzer KA, Wier GM, Dam RA, Srinivasan AR, Borges AL, English ED, Herrmann DC, Schares G, Dubey JP and Boyle JP. Hammondia hammondi harbors functional orthologs of the host-modulating effectors GRA15 and ROP16 but is distinguished from Toxoplasma gondii by a unique transcriptional profile. Eukaryot Cell.  13(12):1507-18. doi: 10.1128/EC.00215-14. Epub 2014 Oct 3.
Bayer A, Delorme-Axford E, Sleigher C, Frey TK, Trobaugh DW, Klimstra WB, Emert-Sedlak LA, Smithgall TE, Kinchington PR, Vadia S, Seveau S, Boyle JP, Coyne CB, Sadovsky Y.  (2014)  Human trophoblasts confer resistance to viruses implicated in perinatal infection.  American Journal of Obstetrics and Gynecology.  Aug 6. pii: S0002-9378(14)00809-6.

Morgado P, Sudarshana D, Gov L, Harker K, Lam T, Casali P, Boyle JP, and Lodoen M.  (2014).  Type II Toxoplasma gondii induction of CD40 on infected macrophages enhances IL-12 responses.  Infection and Immunity. Jul 14. pii: IAI.01615-14.


Pernas L, Adomako-Ankomah Y, Shastri AJ, Ewald SE, Treeck M, Boyle JP, Boothroyd JC. (2014) Toxoplasma effector MAF1 mediates recruitment of host mitochondria and impacts the host response. PLoS Biology. 12(4):e1001845.  Link to open access article.


Adomako-Ankomah Y, Wier GM, Borges AL, Wand HE and Boyle JP.  (2014) Differential locus expansion distinguishes Toxoplasmatinae species and closely related strains of Toxoplasma gondii.    MBio 5(1):e01003-13.  Link to open access article.

Latorraca NR, Callenberg KM, Boyle JP and Grabe M.  Continuum approaches to understanding ion and Peptide interactions with the membrane.  (2014)  Journal of Membrane Biology. May;247(5):395-408.  Link to article.

Walzer KA, Adomako-Ankomah Y, Dam RA, Herrmann DC, Schares G, Dubey JP, and Boyle JP. (2013) Hammondia hammondi, an avirulent relative of Toxoplasma gondii, has functional orthologs of known T. gondii virulence genes. Proc Natl Acad Sci U S A 110:7446-7451.  Link to article.


Feliu V, Vasseur V, Grover HS, Chu HH, Brown MJ, Wang J, Boyle JP, Robey EA, Shastri N, Blanchard N. (2013) Location of the CD8 T cell epitope within the antigenic precursor determines immunogenicity and protection against the Toxoplasma gondii parasite.  PLoS Pathogens.  9(6):e1003449.  


Dubey JP, Tilahun G, Boyle JP, Schares G, Verma SK, Ferreira LR, Oliveira S, Tiao N, Darrington C, and Gebreyes WA. (2013) Molecular and Biological Characterization of First Isolates of Hammondia hammondi from Cats from Ethiopia. Journal of Parasitology. 99:614-618.  Link to article.


Kamau ET, Srinivasan AR, Brown MJ, Fair MG, Caraher EJ, and Boyle JP. (2012) A focused small-molecule screen identifies 14 compounds with distinct effects on Toxoplasma gondii. Antimicrobial Agents & Chemotherapy 56:5581-5590.

Opinion piece:  Reese ML and Boyle JP. (2012) Virulence without catalysis: How can a pseudokinase affect host cell signaling?  Trends in Parasitology.  Feb;28(2):53-7.

Wasmuth JD, Pszenny V, Haile S, Jansen EM, Gast AT, Sher A, Boyle JP, Boulanger MJ, Parkinson J, and Grigg ME. (2012) Integrated bioinformatic and targeted deletion analyses of the SRS gene superfamily identify SRS29C as a negative regulator of Toxoplasma virulence. mBio 3(6).

Commentary:  Walzer KA and Boyle JP. (2012) A single chromosome unexpectedly links highly divergent isolates of Toxoplasma gondii. mBio 3(1):e00284-11.
2011 Kamau E, Meehan T, Lavine MD, Arrizabalaga G, Mustata Wilson G, and Boyle JP. (2011) A novel benzodioxole-containing inhibitor of Toxoplasma gondii growth alters the parasite cell cycle. Antimicrobial Agents & Chemotherapy 55:5438-5451.

Reese ML, Zeiner GM, Saeij JP, Boothroyd JC, and Boyle JP. (2011) Polymorphic family of injected pseudokinases is paramount in Toxoplasma virulence. Proc Natl Acad Sci U S A.  108:9625-9630.


Ong YC, Boyle JP, and Boothroyd JC. (2011) Strain-dependent host transcriptional responses to Toxoplasma infection are largely conserved in mammalian and avian hosts. PLoS ONE.  6:e26369.

Review:  Adomako-Ankomah Y, Wier G and Boyle JP.  (2011)  Beyond the genome:  Recent advances in Toxoplasma gondii functional genomics.  Parasite Immunology.  55(12):5438-51.

Khaminets A, Hunn JP, Konen-Waisman S, Zhao YO, Preukschat D, Coers J, Boyle JP, Ong YC, Boothroyd JC, Reichmann G, and Howard JC. (2010) Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole. Cellular Microbiology.  12:939-961.


Bontell IL, Hall N, Ashelford KE, Dubey JP, Boyle JP, Lindh J, and Smith JE. (2009) Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variants. Genome Biology 10:R53.

Review:  Boyle JP and Radke J. (2009)  A brief history of studies that examine the interactions of Toxoplasma with its host cell:  Emphasis on in vitro models.  International Journal for Parasitology. 39:903-914.

Boyle JP, Saeij JP, Harada SY, Ajioka JW, and Boothroyd JC. (2008) Expression quantitative trait locus mapping of Toxoplasma genes reveals multiple mechanisms for strain-specific differences in gene expression. Eukaryotic Cell.  7:1403-1414.


Boyle JP, Saeij JP, and Boothroyd JC. (2007) Toxoplasma gondii: inconsistent dissemination patterns following oral infection in mice. Experimental Parasitology.  116:302-305.


Saeij JP, Coller S, Boyle JP, Jerome ME, White MW, and Boothroyd JC. (2007) Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. Nature.  445:324-327.


Boyle JP, Rajasekar B, Saeij JP, Ajioka JW, Berriman M, Paulsen I, Roos DS, Sibley LD, White MW, and Boothroyd JC. (2006) Just one cross appears capable of dramatically altering the population biology of a eukaryotic pathogen like Toxoplasma gondii. Proc Natl Acad Sci U S A.  103:10514-10519.


Saeij JP, Boyle JP, Coller S, Taylor S, Sibley LD, Brooke-Powell ET, Ajioka JW, and Boothroyd JC. (2006) Polymorphic secreted kinases are key virulence factors in toxoplasmosis. Science.  314:1780-1783.

Review:  Boyle JP, Saeij JPJ, Cleary MD and Boothroyd JC. (2006)  Analysis of gene expression during development: lessons from the Apicomplexa.  Microbes and Infection. 8(6):1623-30.
2005 Boyle JP, and Yoshino TP. (2005) Serotonin-induced muscular activity in Schistosoma mansoni larval stages: importance of 5-HT transport and role in daughter sporocyst production. Journal of Parasitology.  91:542-550.

Hillyer JF, Schmidt SL, Fuchs JF, Boyle JP, and Christensen BM. (2005) Age-associated mortality in immune challenged mosquitoes (Aedes aegypti) correlates with a decrease in haemocyte numbers. Cellular Microbiology.  7:39-51.


Khan A, Taylor S, Su C, Mackey AJ, Boyle J, Cole R, Glover D, Tang K, Paulsen IT, Berriman M, Boothroyd JC, Pfefferkorn ER, Dubey JP, Ajioka JW, Roos DS, Wootton JC, and Sibley LD. (2005) Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii. Nucleic Acids Research.  33:2980-2992.


Saeij JP, Boyle JP, Grigg ME, Arrizabalaga G, and Boothroyd JC. (2005) Bioluminescence imaging of Toxoplasma gondii infection in living mice reveals dramatic differences between strains. Infection and Immunity.  73:695-702.

Review:  Saeij JPJ, Boyle JP and Boothroyd JC. (2005)  Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host.  Trends in Parasitology.  21(10):476-81.

Bartholomay LC, Cho WL, Rocheleau TA, Boyle JP, Beck ET, Fuchs JF, Liss P, Rusch M, Butler KM, Wu RC, Lin SP, Kuo HY, Tsao IY, Huang CY, Liu TT, Hsiao KJ, Tsai SF, Yang UC, Nappi AJ, Perna NT, Chen CC, and Christensen BM. (2004) Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus. Infection and Immunity.  72:4114-4126.


Vermeire JJ, Boyle JP, and Yoshino TP. (2004) Differential gene expression and the effects of Biomphalaria glabrata embryonic (Bge) cell factors during larval Schistosoma mansoni development. Molecular and Biochemical Parasitology.  135:153-157.


Boyle JP, Hillyer JF, and Yoshino TP. (2003) Pharmacological and autoradiographical characterization of serotonin transporter-like activity in sporocysts of the human blood fluke, Schistosoma mansoni. Journal of Comparative Physiology A.  12:12.


Boyle JP, Wu XJ, Shoemaker CB, and Yoshino TP. (2003) Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts. Molecular and Biochemical Parasitology.  128:205-215.

Review:  Boyle JP and Yoshino TP (2003) Gene manipulation of parasitic helminths.  International Journal for Parasitology.  33(11):1259-1268.

Boyle JP, and Yoshino TP. (2002) Monoamines in the albumen gland, plasma, and central nervous system of the snail Biomphalaria glabrata during egg-laying. Comparative Biochemistry and Physiology A.  132:411-422.

2001 Review:  Yoshino TP, Boyle JP and Humphries JE (2001)  Receptor-ligand interactions and cellular signaling at the host-parasite interface. Parasitology, 123:S143-157.
2000 Boyle JP, Zaide JV, and Yoshino TP. (2000) Schistosoma mansoni: effects of serotonin and serotonin receptor antagonists on motility and length of primary sporocysts in vitro. Experimental Parasitology.  94:217-226.