Recipes
Buffer Recipes
- Homogenization Buffer
- Buffer C (Sepharose 4B Column Buffer)
- Buffer D (Cytosol Dialysis Buffer)
- 10 X Golgi Assay Buffer Mix (Minus DTT)
- 10 X Synaptosome Assay Buffer Mix (Minus DTT)
- GTP Overlay Buffer
- 4 X SDS Sample Buffer
- 2-D Gel Equilibration Buffer
- 10 X KHMgE (Heuser) Buffer
Gel Recipes
- 30:0.4 Acrylaminde/bis-acrylaminde Stock
- 4X Concentrated Running Gel Buffer
- 4X Concentrated Stacking Gel Buffer
- 10X Concentrated SDS-PAGE Reservoir Buffer
- 10X Concentrated Immunoblotting TRANSFER Buffer
- 10 X Concentrated Tris-buffered saline + Tween (TBST)
- 6.5% gel (8 ml)
- 6.5% gel (20 ml)
- 8.0% gel (20 ml)
- 10.0% gel (20 ml)
- 11.0 % gel (20 ml)
- 12.5 % gel (20 ml)
- 14.0% gel (20 ml)
- 4.0% Stacking Gel (5 ml)
Back to Top
-----------------------
Preparations
Rat Brain Synaptosomes Preparation
- Synaptosome Homogenization Buffer
- 6% (w/v) FICOLL 400 In Homogenization Buffer
- 9% (w/v) FICOLL 400 In Homogenization Buffer
- 13% (w/v) FICOLL 400 In Homogenization Buffer
Rat Liver Lysosomes Preparation
Synaptic Plasma Membranes from Gradient-Purified Synaptosomes Preparation
- Hypotonic Synaptosome Lysis Buffer
- 1.7 M Sucrose Solution (48% w/w)
- 0.92 M Sucrose Solution (28.5% w/w)
Back to Top
|
